Analysis of mines and contaminated agricultural soil samples for fungal diversity and tolerance to heavy metals

Urban Farm DB summary

Soil samples were collected from peri-urban agricultural land in Lahore, Pakistan, irrigated by industrial and sewerage waste from the Hudiara drain, and from mine waste soil in Wiesloch, Germany, and analyzed for heavy metals by X-ray fluorescence and ICP-MS and for fungal diversity and frequency by a soil dilution method. X-ray fluorescence found higher Zn, As and Pb in the German mine waste soil, and the more sensitive ICP-MS found higher Fe, Mn, Cu, As, Sr, Mo, Cd, Sb, Ti, Zn and Pb there as well; the Pakistani peri-urban soils had lower heavy metal concentrations than the German mine waste but higher overall fungal frequency and diversity. Testing radial growth of isolated Aspergillus niger, A. flavus and A. nodulans against CdCl2, CuSO4, NiCl2 and ZnCl2 at 1-40 ppm, all tested fungi tolerated ZnCl2 (25 ppm) and NiCl2 (12 ppm) but none tolerated CdCl2 or CuSO4.