Collection of biospecimens from parent-child dyads in a community garden-based nutrition intervention: protocol and feasibility

Urban Farm DB summary

Non-invasive human biospecimens including stool, urine, and hair are important for understanding the relationship between diet and changes in human physiologic processes affecting chronic disease outcomes, but collection can be difficult for studies conducted away from a centralized location. This paper describes the protocol and feasibility of collecting stool, urine, and hair biospecimens from parents and children at a remote location, as part of the Summer Harvest Adventure (SHA) study, a randomized controlled, community garden-based intervention targeting children aged 8-11 and their parents from low-resource neighborhoods in the United States. Biospecimens were collected at baseline and post-intervention from willing children and parents in both intervention and control groups, for microbiome, metabolomics, and hair analyses, at a location distant from the academic laboratories conducting the analysis. Of 146 SHA participants (73 parent-child dyads) eligible to provide a biospecimen, 126 consented to hair, 115 to stool, and 127 to urine collection. Among those who consented, 44 children (69.8%) and 38 parents (60.3%) provided at least one hair sample; 27 children (48.2%) and 37 parents (62.7%) provided at least one stool sample; and 36 children (57.1%) and 42 parents (65.6%) provided at least one urine sample. Sample collection, transport, and handling at the academic center were successful, and all biospecimens collected were deemed adequate for analysis. DNA and metabolomics yield on a subset of stool samples showed an abundance of species and metabolites as predicted. Urine and hair analyses were described as still underway, with the study characterized as among the first to describe the feasibility of collecting these biospecimens from both parents and children in this remote setting.