Genotyping of Giardia duodenalis in vegetables cultivated with organic and chemical fertilizer from street markets and community vegetable gardens in a region of Southern Brazil

Urban Farm DB summary

This study genotyped Giardia duodenalis in raw-eaten vegetables (crisp lettuce, regular lettuce, kale, chicory, and rocket) sampled from street markets and community vegetable gardens in an urban area of southern Brazil. Researchers analyzed 130 samples from street markets and 130 from community gardens (260 total), washing them in Tween 80 solution, filtering through a cellulose acetate membrane, amplifying the GDH gene by semi-nested PCR, and genotyping positive samples by PCR-RFLP. 7.3% (19/260) of samples tested positive for G. duodenalis, from both street markets (10/130) and community gardens (9/130), including both organic and non-organic products. Assemblage AI predominated, but assemblages B and E, which carry zoonotic potential, were also found. The study concludes this points to a need for investigating commercially sold raw vegetables and establishing stricter quality control.