Molecular biodiversity of arbuscular mycorrhizal fungi in trace metal‐polluted soils

Urban Farm DB summary

A study assessing indigenous arbuscular mycorrhizal fungi (AMF) community structure in roots and soil of Plantago major (plantain) growing on trace-metal (TM)-contaminated versus uncontaminated sites, selected based on a survey of TM concentrations (As, Cd, Cu, Pb, Sn, Zn) in community garden soils in Montreal, Canada. AMF community structure was analyzed using PCR-DGGE, cloning and sequencing, and direct sequencing. Native AMF diversity, measured by the number of AMF ribotypes, was decreased in TM-polluted soils and plant roots compared with unpolluted sites, and community structure was altered by TM contamination. Various Glomus species along with Scutellospora aurigloba and S. calospora were the most abundant ribotypes in unpolluted soil, while G. etunicatum, G. irregulare/G. intraradices and G. viscosum occurred in both polluted and unpolluted soils, and G. mosseae together with unidentified Glomus ribotypes (B9, B13) dominated in TM-polluted sites. The predominance of G. mosseae at metal-polluted sites is interpreted as suggesting tolerance to TM stress and potential use for phytoremediation.